Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020213_mivi GGTGCACAGGCCTCGGAAGGCAGAGGCAGGGG(+ACTCAC)GATCCCTACCGGTTTAGAATCTGCATGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605020211_wd4x TATGCAGCCCATGCAGATTCTAAACCGGTAGG(+ACTCAC)GATCCCCCTGCCTCTGCCTTCCGAGGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605020209_q2h9 TGTCTCGGTCCCGGCCGCTGCTCTCCAGCCAG(+ACTCAC)GTACCCTCCTCACTCCCTGCTCGCGCAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605020207_1pw4 AGTGCGAACTGCGCGAGCAGGGAGTGAGGAGG(+ACTCAC)GTACCTGGCTGGAGAGCAGCGGCCGGGACCG NGG 10 8 17 8 24 0 100 5 10
    240605020205_embe GGCGCCGGGGGCGGTGCCGGCTCCAATCACGG(+ACTCAC)GATCCGCCCAAGGGCCCCAACGCGGTAGGCG NGG 10 8 17 8 24 0 100 5 10
    240605020204_971f GGAAGCGCCTACCGCGTTGGGGCCCTTGGGCG(+ACTCAC)GATCCCGTGATTGGAGCCGGCACCGCCCCCG NGG 10 8 17 8 24 0 100 5 10
    240605020202_mfio TCCTTCTTGCTCCGCTTCTATTCTGTCAAGGG(+ACTCAC)GATCCCTGCAGTCTGAGGCTTCCCCCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605020200_f256 CCAGGTTTGGGGGGGAAGCCTCAGACTGCAGG(+ACTCAC)GATCCCCTTGACAGAATAGAAGCGGAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605020159_vmts TGGGTCCCAATGCATCTGCTGGGGGCTGAGAG(+ACTCAC)GTACCTTCTGCCCATCACCAGCCTTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605020158_hqr3 CAGCACAGAACAAGGCTGGTGATGGGCAGAAG(+ACTCAC)GTACCTCTCAGCCCCCAGCAGATGCATTGGG NGG 10 8 17 8 24 0 100 5 10