Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020316_fi6p GCCCTCTTTCCTTGGCACATTCCATGTAGTTG(+ACTCAC)GTACCCTTGTGGGGACCCACAGCATCCATGG NGG 10 8 17 8 24 0 100 5 10
    240605020315_x8rr GGTTTCCATGGATGCTGTGGGTCCCCACAAGG(+ACTCAC)GTACCAACTACATGGAATGTGCCAAGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605020313_ww5w ATGCAGGTTAAGTGCCTAAGGTTTCCTGTTGG(+ACTCAC)GTACCACATGCTTCTCTAAAAGGCTTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605020311_1c4h GACTCCCTTAAAGCCTTTTAGAGAAGCATGTG(+ACTCAC)GTACCCAACAGGAAACCTTAGGCACTTAACC NGG 10 8 17 8 24 0 100 5 10
    240605020310_xm22 CACTAACTACTGTGACAAGAAACTTAGCAAAG(+ACTCAC)GTACCTGTGTGTACAGTGAGGGCAGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605020309_0hoh CTCATAGCCAGCTGCCCTCACTGTACACACAG(+ACTCAC)GTACCTTTGCTAAGTTTCTTGTCACAGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605020307_tfzq AGTCGTCCCTCTGTCAGCTTGGAGCGTCTCAG(+ACTCAC)GTACCCAGGCAGTCTCAGACTTCTGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605020305_b3ul TTTCGGAGGCCCAGAAGTCTGAGACTGCCTGG(+ACTCAC)GTACCTGAGACGCTCCAAGCTGACAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605020303_cs55 GAGGTGAGCGGGGCTGATCTATACCAAGCCAG(+ACTCAC)GATCCTTCTGAATTAGATTCTTGTCTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605020302_er9v AACTGTCCCAAGACAAGAATCTAATTCAGAAG(+ACTCAC)GATCCTGGCTTGGTATAGATCAGCCCCGCTC NGG 10 8 17 8 24 0 100 5 10