Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020435_od0g CAAAGAGAAGTAAAGGGATTTGTCTCCATTGG(+ACTCAC)GTACCAGACCACCATTGCGTTGACCTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605020433_d45w CATGTCTCTTAAGTTATTGTGACCTACACTGG(+ACTCAC)GTACCGGAGGGGATGGGGGATGGCTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605020430_e48q ACAGCAGCTGAAGCCATCCCCCATCCCCTCCG(+ACTCAC)GTACCCAGTGTAGGTCACAATAACTTAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605020429_hvv8 TGTGGCACTCAAGCATACTGATCCCCGCTCAG(+ACTCAC)GTACCACCATTCCCTATGTAGAGGAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605020427_25d2 GGGTGCTTCCCTCCTCTACATAGGGAATGGTG(+ACTCAC)GTACCTGAGCGGGGATCAGTATGCTTGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605020426_o5hm ATAGAACACTCAAAAGGGACGTGAATGTGCAG(+ACTCAC)GTACCATGGGTCTGGGGTGACCTGCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240605020423_isqr ACTTCTGTGAAGCAGGTCACCCCAGACCCATG(+ACTCAC)GTACCTGCACATTCACGTCCCTTTTGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605020422_b79z GCTGCCTGAGGGAGCCTTTGGAGAGCTAACTG(+ACTCAC)GTACCCTGATTATAGTCCATTAAGAATCACT NGG 10 8 17 8 24 0 100 5 10
    240605020421_klgz ACCTAAGTGATTCTTAATGGACTATAATCAGG(+ACTCAC)GTACCAGTTAGCTCTCCAAAGGCTCCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605020419_1fjl AGACACAGGTGCAGAGCGGGAAGGCTGACTGG(+ACTCAC)GATCCCGCCACCAGCTGTTTTCCCTCTTGTC NGG 10 8 17 8 24 0 100 5 10