Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014626_7whn AAAACACATTTAAAGGGACAGAAGGAGCGCTG(+ACTCAC)GATCCTGGCTTTTGACCGGAAGGCTCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605014624_lo0x TGCTGTTCCAGAGCCTTCCGGTCAAAAGCCAG(+ACTCAC)GATCCAGCGCTCCTTCTGTCCCTTTAAATGT NGG 10 8 17 8 24 0 100 5 10
    240605014623_4xlj TCTTATCTTCCCCACCTTCAAACCCCTCTCAG(+ACTCAC)GTACCCCACATGCCCACTTCTCTGGCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605014621_k2dv CAGAAAGGGAGCCAGAGAAGTGGGCATGTGGG(+ACTCAC)GTACCTGAGAGGGGTTTGAAGGTGGGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605014619_3ec2 TGATGGGGGACAGTATGAAAAGAAAGAACAAG(+ACTCAC)GTACCAACAGTTTTATCTATCAAAACTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605014618_8v9o GTGATATTTAGTTTTGATAGATAAAACTGTTG(+ACTCAC)GTACCTTGTTCTTTCTTTTCATACTGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605014617_w5d2 TGTGCACTATCCATGTGACCGAAGGACACATG(+ACTCAC)GATCCTACCGCTAAGCCCTTCCTGATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605014616_t1e6 TGGCCTGGGCATCAGGAAGGGCTTAGCGGTAG(+ACTCAC)GATCCATGTGTCCTTCGGTCACATGGATAGT NGG 10 8 17 8 24 0 100 5 10
    240605014614_t33y ATCTTCTGAATCCCTGTTTACAAATGCTGCAG(+ACTCAC)GATCCTTTCATGAATCAGAATGAGCACAGAT NGG 10 8 17 8 24 0 100 5 10
    240605014613_k51b CCCTCATCTGTGCTCATTCTGATTCATGAAAG(+ACTCAC)GATCCTGCAGCATTTGTAAACAGGGATTCAG NGG 10 8 17 8 24 0 100 5 10