Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014555_qorc TGTGGGAAGGGGTGTCGGCATACAGATCAGTG(+ACTCAC)GTACCACCCACCTTCTGATGTAACATGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605014553_vf9e GAGTGGGAACATGTTACATCAGAAGGTGGGTG(+ACTCAC)GTACCACTGATCTGTATGCCGACACCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605014551_5hmk CCTACCTCCCAGCACGGCTGTCCTCACTACGG(+ACTCAC)GTACCAGAGCTGTGGTTTCCATAGCACAGCA NGG 10 8 17 8 24 0 100 5 10
    240605014550_bvc3 TTTCTTGCTGTGCTATGGAAACCACAGCTCTG(+ACTCAC)GTACCCGTAGTGAGGACAGCCGTGCTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605014549_cbdx CGCTGAATGTACCTGTGGCTGCAGAGGACCTG(+ACTCAC)GTACCCTCCTCTGAAAGCTCCATCTTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605014547_z4rn CCACAAGGCCAAGATGGAGCTTTCAGAGGAGG(+ACTCAC)GTACCAGGTCCTCTGCAGCCACAGGTACATT NGG 10 8 17 8 24 0 100 5 10
    240605014546_ly0q TTCTCCTTGTCTGTGGTGACCAGGGTGTAAAG(+ACTCAC)GTACCTGCTGCAGCTAACCTTGAACTTCACA NGG 10 8 17 8 24 0 100 5 10
    240605014544_vx6e GATAATGTGAAGTTCAAGGTTAGCTGCAGCAG(+ACTCAC)GTACCTTTACACCCTGGTCACCACAGACAAG NGG 10 8 17 8 24 0 100 5 10
    240605014543_p2nt GTGTTTTCTGTCCAAAACTCTGAAATAATAAG(+ACTCAC)GATCCCTTAGAGTATGGCAAAACCCCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605014542_4pkd ATTATGTTTGGGGGTTTTGCCATACTCTAAGG(+ACTCAC)GATCCTTATTATTTCAGAGTTTTGGACAGAA NGG 10 8 17 8 24 0 100 5 10