Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014612_8q2j AGTTTCCTAAGCAACTCTAGAAGTGCCACTTG(+ACTCAC)GATCCATGGAATTACACTGACTTCTCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605014610_0ibr GTAAAGGGAAGAGAAGTCAGTGTAATTCCATG(+ACTCAC)GATCCAAGTGGCACTTCTAGAGTTGCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605014609_b2cf TTTTACTCCCAAGACCTCAGGGGGAGTAACTG(+ACTCAC)GTACCCAAAGTACCACTTGGTCTGAGCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605014607_jf67 AGAGAGAAGGCTCAGACCAAGTGGTACTTTGG(+ACTCAC)GTACCAGTTACTCCCCCTGAGGTCTTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605014606_bbbu AGTCTTGGGAGAGGCTCAGGATGGAATTTTGG(+ACTCAC)GATCCCTGGGGTCTTTGGTACGGAGGATAAG NGG 10 8 17 8 24 0 100 5 10
    240605014603_jnsb GTCACCTTATCCTCCGTACCAAAGACCCCAGG(+ACTCAC)GATCCCAAAATTCCATCCTGAGCCTCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605014601_lt9q GGAAAGTGGGCCATAGAGGGGAAGGCCACAGG(+ACTCAC)GATCCTGCTGAGAGCCCTGCCTTACATAATG NGG 10 8 17 8 24 0 100 5 10
    240605014600_okdz GCAGACATTATGTAAGGCAGGGCTCTCAGCAG(+ACTCAC)GATCCCTGTGGCCTTCCCCTCTATGGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605014559_e7dg TTCCATTCTCTATGTTGTTAGTAAATACTTTG(+ACTCAC)GATCCCGGCTAGTGTTCTCTACCCTACTCCC NGG 10 8 17 8 24 0 100 5 10
    240605014557_m6qu CAGAGGGGAGTAGGGTAGAGAACACTAGCCGG(+ACTCAC)GATCCAAAGTATTTACTAACAACATAGAGAA NGG 10 8 17 8 24 0 100 5 10