Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014452_2vsb AACCTGAATTTCATCCTCGGGACTCAATAGTG(+ACTCAC)GATCCAGAGAACCAACTTCTAAAACTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605014450_6404 AGTCAGGACAAGTTTTAGAAGTTGGTTCTCTG(+ACTCAC)GATCCACTATTGAGTCCCGAGGATGAAATTC NGG 10 8 17 8 24 0 100 5 10
    240605014449_a9ow GTGTGTGCATTTACCCACAGAACAAGGCATTG(+ACTCAC)GATCCCTTGGCACTGGTGTCTCTGTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605014447_sn6c GAGACAGAGAGACAGAGACACCAGTGCCAAGG(+ACTCAC)GATCCAATGCCTTGTTCTGTGGGTAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605014445_i7uw TGTTCTCGGCATTAGTTTTCAAAATGTCCATG(+ACTCAC)GATCCCTCGTCATCTGAGGAGCTGATTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605014444_sexi CCCTCCTACAATCAGCTCCTCAGATGACGAGG(+ACTCAC)GATCCATGGACATTTTGAAAACTAATGCCGA NGG 10 8 17 8 24 0 100 5 10
    240605014442_ojvw TATGAAAATGGAGGGTAGACAACAGGTATCAG(+ACTCAC)GTACCCACAAAATGAACCTCACCATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605014441_14qn TGTGGTACTTCATGGTGAGGTTCATTTTGTGG(+ACTCAC)GTACCTGATACCTGTTGTCTACCCTCCATTT NGG 10 8 17 8 24 0 100 5 10
    240605014438_apmn GTTCTGAAGTAGATGGTTGCCATGAACTCCCG(+ACTCAC)GATCCAGATAGTCTCATTCATGATGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605014437_4emk GGGATCACCTCCATCATGAATGAGACTATCTG(+ACTCAC)GATCCGGGAGTTCATGGCAACCATCTACTTC NGG 10 8 17 8 24 0 100 5 10