Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014406_p7oq TTGTTGAAGCTGCTGGCCACCAGCAATGATAG(+ACTCAC)GATCCAATGGCGAGTTGGGCCGTATCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605014404_mjmx ATGGGTCTCAGATACGGCCCAACTCGCCATTG(+ACTCAC)GATCCTATCATTGCTGGTGGCCAGCAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605014403_9bsp CAACATTTCAGTAAGTATCCCTATATGACCTG(+ACTCAC)GATCCCAAGCACATCTTAAGGCTCTACACCT NGG 10 8 17 8 24 0 100 5 10
    240605014401_5vsd TATGTAGGTGTAGAGCCTTAAGATGTGCTTGG(+ACTCAC)GATCCAGGTCATATAGGGATACTTACTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605014359_qyjf TCTGAGTTCTGATGTCTCTAAGTCCTTATTGG(+ACTCAC)GTACCGGGGAGCTGCAGGAGCAGAGGCAACG NGG 10 8 17 8 24 0 100 5 10
    240605014356_t8yt GGCTGCGTTGCCTCTGCTCCTGCAGCTCCCCG(+ACTCAC)GTACCCAATAAGGACTTAGAGACATCAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605014354_wtbk ATCCACCATGTGGGTGCTGGAATCGAACCCAG(+ACTCAC)GTACCCTGGAAGAGCAGCCACTATTCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605014352_drcu CAGCAGTTAAGAATAGTGGCTGCTCTTCCAGG(+ACTCAC)GTACCTGGGTTCGATTCCAGCACCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240605014351_eao2 GCACTTGCCACCAAGTCTAGAGACTTGAGTTG(+ACTCAC)GATCCCCAGGACTAACTTTGTTGGAAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605014348_i7jz AGTCAGTTCTTTCCAACAAAGTTAGTCCTGGG(+ACTCAC)GATCCAACTCAAGTCTCTAGACTTGGTGGCA NGG 10 8 17 8 24 0 100 5 10