Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014436_kdy1 AATGAAAAGCAGCTAACTACAGAAGCAACATG(+ACTCAC)GATCCTGCTCTGATTACCCATCGTCCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605014435_48qm TCAGCTCTAGGGACGATGGGTAATCAGAGCAG(+ACTCAC)GATCCATGTTGCTTCTGTAGTTAGCTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605014433_banm CAGGATGAGAACAGGCTGGGATGAACTCAGAG(+ACTCAC)GTACCAGATGAGCTTGATCTTGAAGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605014432_8fy7 TGAGTCAGACTCTTCAAGATCAAGCTCATCTG(+ACTCAC)GTACCTCTGAGTTCATCCCAGCCTGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605014430_ee03 GTTTCACAACGCTGAAAATAAGGTGGCAGGTG(+ACTCAC)GTACCCTTCCTTCCCAGAAGGAAAGAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605014429_29yy TCTTCTTCTCTCTTTCCTTCTGGGAAGGAAGG(+ACTCAC)GTACCACCTGCCACCTTATTTTCAGCGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605014427_nyqn AGCCACCTTATGGATGCTGAGAGTGTAAGCTG(+ACTCAC)GATCCTCTACAAGAGCAGCCAGAGCTCTTAT NGG 10 8 17 8 24 0 100 5 10
    240605014426_evl8 AATGGATAAGAGCTCTGGCTGCTCTTGTAGAG(+ACTCAC)GATCCAGCTTACACTCTCAGCATCCATAAGG NGG 10 8 17 8 24 0 100 5 10
    240605014425_e9qa TACTGGCAACGTGTAAATAGTGTTGGTACACG(+ACTCAC)GATCCAGGCTGTACTGTAAGCACAAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240605014423_b5nb ATGTTCAAAATTTGTGCTTACAGTACAGCCTG(+ACTCAC)GATCCGTGTACCAACACTATTTACACGTTGC NGG 10 8 17 8 24 0 100 5 10