Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014331_tfam TAGAAAATGCTGCAGATACCTGTGGAGTTGTG(+ACTCAC)GATCCAGGCATGACTCTAGGGTAGAAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605014330_i3yp GGAAAAGCCCTTCTACCCTAGAGTCATGCCTG(+ACTCAC)GATCCACAACTCCACAGGTATCTGCAGCATT NGG 10 8 17 8 24 0 100 5 10
    240605014328_1kzx AGGCAGTTAGGAACTGCTCATTGTCGTGTTGG(+ACTCAC)GTACCAAACTTGGGTCTTTTGAAAGACCAGT NGG 10 8 17 8 24 0 100 5 10
    240605014327_szvo TATAAACTGGTCTTTCAAAAGACCCAAGTTTG(+ACTCAC)GTACCCAACACGACAATGAGCAGTTCCTAAC NGG 10 8 17 8 24 0 100 5 10
    240605014325_kfau ATGGGCATGGGGCCATCTCTTCAGTCTAGATG(+ACTCAC)GTACCCTCTGCACTCTGCCGCCATCTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605014324_rif5 TGCATCCACTAGATGGCGGCAGAGTGCAGAGG(+ACTCAC)GTACCATCTAGACTGAAGAGATGGCCCCATG NGG 10 8 17 8 24 0 100 5 10
    240605014322_eivc TCCTGGCTAGGAGATGGCAGTGCTGAGCTAGG(+ACTCAC)GATCCTTAGCAGGACCCAGGCTTGTCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605014321_j3tt AGGTGAGGAAGACAAGCCTGGGTCCTGCTAAG(+ACTCAC)GATCCCTAGCTCAGCACTGCCATCTCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605014319_yjay CCTCCTGAGAGCCAGGATATAGTTTACTGGGG(+ACTCAC)GATCCGTGAAGAATTCGGTTCCTGTCCTGAC NGG 10 8 17 8 24 0 100 5 10
    240605014318_b8ql GCTAGGTCAGGACAGGAACCGAATTCTTCACG(+ACTCAC)GATCCCCCAGTAAACTATATCCTGGCTCTCA NGG 10 8 17 8 24 0 100 5 10