Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010326_ihng GGTCTGTCATGGCACGCCACACTGACTGGTGG(+ACTCAC)GATCCTTACAGGAAACTCCAATCCCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605010324_2w6w TGCTGCACACTGGGATTGGAGTTTCCTGTAAG(+ACTCAC)GATCCCACCAGTCAGTGTGGCGTGCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240605010323_3bty TTGTTTGGTTCTTGTAGGCTGGTTGGTTTCAG(+ACTCAC)GTACCTGAGACAATAGCACCAAATTCAACAG NGG 10 8 17 8 24 0 100 5 10
    240605010322_g3ac TTTCTCTGTTGAATTTGGTGCTATTGTCTCAG(+ACTCAC)GTACCTGAAACCAACCAGCCTACAAGAACCA NGG 10 8 17 8 24 0 100 5 10
    240605010320_q445 TCTAGCCCACAGCTCCTTGCCTGTTGGACCTG(+ACTCAC)GATCCTTTCAGGCTTGTACCCCAGACTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605010319_0fxb AGGTGGAGCAGTCTGGGGTACAAGCCTGAAAG(+ACTCAC)GATCCAGGTCCAACAGGCAAGGAGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605010317_oh83 GGGCCGGGGGCACACCACTCAGAGAAAGCAGG(+ACTCAC)GTACCTGTCTGGCATGTTCTGGATAGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605010316_n4yj GGGCTCAGACCTATCCAGAACATGCCAGACAG(+ACTCAC)GTACCCTGCTTTCTCTGAGTGGTGTGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240605010314_28q7 ATCCAGACAGTCCTGAGAGCGGAAGTGCTCGG(+ACTCAC)GATCCCTGCTACCTCAGGACCTGGATTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605010312_fwgy CAGCCTTTTAATCCAGGTCCTGAGGTAGCAGG(+ACTCAC)GATCCCGAGCACTTCCGCTCTCAGGACTGTC NGG 10 8 17 8 24 0 100 5 10