Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010220_exs0 ACTGCATTGGTAGGGTACAAAGGTGTTCAGAG(+ACTCAC)GATCCCAGTCTGAGTGATTAGCCTAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605010219_7eza TGCTTCAGTCTTAGGCTAATCACTCAGACTGG(+ACTCAC)GATCCTCTGAACACCTTTGTACCCTACCAAT NGG 10 8 17 8 24 0 100 5 10
    240605010217_hkee CTCCCACGACCTCGGTCCCAGTTGGACACCAG(+ACTCAC)GTACCTCTGGAAAATGAGACAACCGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605010215_94ot CACGCACAGCCCGGTTGTCTCATTTTCCAGAG(+ACTCAC)GTACCTGGTGTCCAACTGGGACCGAGGTCGT NGG 10 8 17 8 24 0 100 5 10
    240605010214_km36 GGTGGAAGGTAGTCGGATCTTCCAGAAGGCTG(+ACTCAC)GATCCTCACGAGGTTGATCAACCTGTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605010213_bu5b CTATCTTTAGACAGGTTGATCAACCTCGTGAG(+ACTCAC)GATCCAGCCTTCTGGAAGATCCGACTACCTT NGG 10 8 17 8 24 0 100 5 10
    240605010211_1lj1 GCTGGGATCATGGATAAGTGTTAACATGCCAG(+ACTCAC)GATCCTCTTCTATCTTTAAGTCTAAGATAGT NGG 10 8 17 8 24 0 100 5 10
    240605010209_m8ro AAGTCACTATCTTAGACTTAAAGATAGAAGAG(+ACTCAC)GATCCTGGCATGTTAACACTTATCCATGATC NGG 10 8 17 8 24 0 100 5 10
    240605010207_xyq3 TGCTGGACCTGGGCAGGATGTACTCCCCTGGG(+ACTCAC)GTACCGGGGCCTGCCTGCGGCTTACCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605010204_l71h TGCAGCAGAAGGTAAGCCGCAGGCAGGCCCCG(+ACTCAC)GTACCCCAGGGGAGTACATCCTGCCCAGGTC NGG 10 8 17 8 24 0 100 5 10