Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010127_1w6t TCTAACATCTAGGTTGAGAACAAGCCTGAATG(+ACTCAC)GTACCCATGGTTGGGAGAGAGAACTTGAACT NGG 10 8 17 8 24 0 100 5 10
    240605010125_0ozt TTTGAAGTTCAAGTTCTCTCTCCCAACCATGG(+ACTCAC)GTACCATTCAGGCTTGTTCTCAACCTAGATG NGG 10 8 17 8 24 0 100 5 10
    240605010124_8zxj ACCCAGGTAATGTGGGCGTGCTCAGAGCGTCG(+ACTCAC)GATCCGGTGGCTGCTTTACACCTGGTGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605010121_he4u TGCTGTTCACACCAGGTGTAAAGCAGCCACCG(+ACTCAC)GATCCGACGCTCTGAGCACGCCCACATTACC NGG 10 8 17 8 24 0 100 5 10
    240605010119_lo3h CCGAGCCAAGTGTCAGATGCACCCTCCACTGG(+ACTCAC)GATCCTCCCCTGAGGATCTAAGGTGTGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605010117_8gxg GTTTCTGCACACACCTTAGATCCTCAGGGGAG(+ACTCAC)GATCCCAGTGGAGGGTGCATCTGACACTTGG NGG 10 8 17 8 24 0 100 5 10
    240605010115_ggsc TCGCGGTGGCCAGGCCCTTGCTCACTCCCTGG(+ACTCAC)GATCCCGATTGAGTTGTCCACCTCTCACTGT NGG 10 8 17 8 24 0 100 5 10
    240605010113_xphd AGGAGACAGTGAGAGGTGGACAACTCAATCGG(+ACTCAC)GATCCCAGGGAGTGAGCAAGGGCCTGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605010111_yu1n GAGAAATGGGGGCACTGCACCCCCACTTCCTG(+ACTCAC)GATCCTGACTCTTAGGCTGGTGGCCCTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605010110_xwv1 GGCGGGGCAAGGGCCACCAGCCTAAGAGTCAG(+ACTCAC)GATCCAGGAAGTGGGGGTGCAGTGCCCCCAT NGG 10 8 17 8 24 0 100 5 10