Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010203_vx07 TTGGGAGCAGTTGTGGAACAGAGGACAGATCG(+ACTCAC)GTACCATACAGAGGCATGGGTTCGCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605010201_mk9i GAGCAGCTACAGCGAACCCATGCCTCTGTATG(+ACTCAC)GTACCGATCTGTCCTCTGTTCCACAACTGCT NGG 10 8 17 8 24 0 100 5 10
    240605010159_4ajq GCTCTCTGAGGGCAACCAGGCCTTGTGTCCTG(+ACTCAC)GTACCTGTGACACTGTTGTGGTCACAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605010158_m47u AGGGCACTGCTGTGACCACAACAGTGTCACAG(+ACTCAC)GTACCAGGACACAAGGCCTGGTTGCCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605010156_h9no AAGCATCAGTTGTGTTCAGAGCTGTGGTCCTG(+ACTCAC)GTACCCCTAGTTGACATCCTGTTTCTGCTTA NGG 10 8 17 8 24 0 100 5 10
    240605010154_9ljw GTACGTAAGCAGAAACAGGATGTCAACTAGGG(+ACTCAC)GTACCAGGACCACAGCTCTGAACACAACTGA NGG 10 8 17 8 24 0 100 5 10
    240605010152_xb9v GGGAGTGAATCGGGGTGAGCAGGCTTTGCCCG(+ACTCAC)GATCCCAGAACTCTAGTCCGTAAGTCCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605010151_tcna TGGTTATGAGGACTTACGGACTAGAGTTCTGG(+ACTCAC)GATCCGGGCAAAGCCTGCTCACCCCGATTCA NGG 10 8 17 8 24 0 100 5 10
    240605010149_dap5 CTTGCTGTGCAGGAGCCACATGGTTCTTCTGG(+ACTCAC)GTACCAGGGCCAGGCCTGGACACCACACAGT NGG 10 8 17 8 24 0 100 5 10
    240605010146_xzuw AACTAACTGTGTGGTGTCCAGGCCTGGCCCTG(+ACTCAC)GTACCCAGAAGAACCATGTGGCTCCTGCACA NGG 10 8 17 8 24 0 100 5 10