Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010311_dl5e TGTGTTTCTCTGCTCTGCTCCGAGGCTAATTG(+ACTCAC)GTACCATGTTACCCCCTTGCCTTGTTACTCT NGG 10 8 17 8 24 0 100 5 10
    240605010310_pf5p GCAGCAGAGTAACAAGGCAAGGGGGTAACATG(+ACTCAC)GTACCAATTAGCCTCGGAGCAGAGCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605010307_6eoi GTCTGCCGAGTTCCTATCATTTCCACTCCCAG(+ACTCAC)GATCCATGGTATTCAGATCAAGTAAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605010305_xq8r CCTCCACAGCCTTACTTGATCTGAATACCATG(+ACTCAC)GATCCTGGGAGTGGAAATGATAGGAACTCGG NGG 10 8 17 8 24 0 100 5 10
    240605010302_864m GCTTCTGCAAATTATCGAAGCTTCTGAGGAAG(+ACTCAC)GATCCATCCAGAGGCTCTGCTTGCCCGCCGC NGG 10 8 17 8 24 0 100 5 10
    240605010301_lzzc CGCAGGCGGCGGGCAAGCAGAGCCTCTGGATG(+ACTCAC)GATCCTTCCTCAGAAGCTTCGATAATTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605010259_gop1 CCAGGCCTCCTGCATCCTGCCCAGACAGGCAG(+ACTCAC)GTACCTGTCTCCCAGTCACAGCTGGGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605010258_rphx CACCTGCACACCCAGCTGTGACTGGGAGACAG(+ACTCAC)GTACCTGCCTGTCTGGGCAGGATGCAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605010257_z11m CAGGCCCAAGACGTCCCAGTGTGCCCCAAATG(+ACTCAC)GATCCTACTTATTTCAAGTCAGCTGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605010255_oyyz TGCCCTGGGCTCAGCTGACTTGAAATAAGTAG(+ACTCAC)GATCCATTTGGGGCACACTGGGACGTCTTGG NGG 10 8 17 8 24 0 100 5 10