Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010108_4m89 ACGTACATTGAGCTCCAGAAGGAAAAGAGAGG(+ACTCAC)GATCCTAACCAAGGGCCACCAGGAAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605010107_hjbg TTGCTTGCTGCTTCCTGGTGGCCCTTGGTTAG(+ACTCAC)GATCCCTCTCTTTTCCTTCTGGAGCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240605010106_y0t3 CAGGAGTCCCTGAGGTTCCCAGCTGCTGTCAG(+ACTCAC)GATCCTGTGAGTTGGGGCAAGCAGGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605010104_7lc4 GGTGGCAGCATCCTGCTTGCCCCAACTCACAG(+ACTCAC)GATCCTGACAGCAGCTGGGAACCTCAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605010103_v7i0 CTGTCACATGCAGCAGACAGGCAGCATTAAGG(+ACTCAC)GTACCCTAGCTCTGTTAGGGCAGAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605010101_pkvu ACATTCCCAGGCTCTGCCCTAACAGAGCTAGG(+ACTCAC)GTACCCTTAATGCTGCCTGTCTGCTGCATGT NGG 10 8 17 8 24 0 100 5 10
    240605010057_wilp GGCGGCACTCGCCCCTCCTGGGGATACAGCAG(+ACTCAC)GATCCGGCCTCAAGAGGCTTCAGGTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605010055_vrda GAGTGTCAAGCACCTGAAGCCTCTTGAGGCCG(+ACTCAC)GATCCTGCTGTATCCCCAGGAGGGGCGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605010053_tfz0 GGTGTAGCCCTACCTACAGTGTGACTAGGCCG(+ACTCAC)GTACCGATCCTGCCTGGTTCTAACCAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605010051_ywky GTCTGTTTTCTGGTTAGAACCAGGCAGGATCG(+ACTCAC)GTACCGGCCTAGTCACACTGTAGGTAGGGCT NGG 10 8 17 8 24 0 100 5 10