Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010050_ylxh AACCCCTTTCAACAGCCCTCCCTCTTCAGTAG(+ACTCAC)GTACCCGCCCCTAGTGATGATGGGCAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605010048_rlw1 CCCACCTGTCTGCCCATCATCACTAGGGGCGG(+ACTCAC)GTACCTACTGAAGAGGGAGGGCTGTTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605010046_pr18 GGTGTAGCCCTACCTACAGTGTGACTAGGCCG(+ACTCAC)GTACCGATCCTGCCTGGTTCTAACCAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605010045_h586 GTCTGTTTTCTGGTTAGAACCAGGCAGGATCG(+ACTCAC)GTACCGGCCTAGTCACACTGTAGGTAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605010043_s9c1 AACCCCTTTCAACAGCCCTCCCTCTTCAGTAG(+ACTCAC)GTACCCGCCCCTAGTGATGATGGGCAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605010041_9ltl CCCACCTGTCTGCCCATCATCACTAGGGGCGG(+ACTCAC)GTACCTACTGAAGAGGGAGGGCTGTTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605010040_grvy AACCTGAAGACCAAAGAGAAGGCAGCCAAGTG(+ACTCAC)GATCCTGGCAGCTGTGGCGCTGCTCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605010038_n85j TACACCTGCAGGAGCAGCGCCACAGCTGCCAG(+ACTCAC)GATCCACTTGGCTGCCTTCTCTTTGGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605010037_zj2x GACAGACAGACAAGGCTGCACGCTCCCTCAAG(+ACTCAC)GATCCCGTCAGGACACTCCTGGCCTACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605010036_kj4s GAATTCAAAGTAGGCCAGGAGTGTCCTGACGG(+ACTCAC)GATCCTTGAGGGAGCGTGCAGCCTTGTCTGT NGG 10 8 17 8 24 0 100 5 10