Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003026_wngg ACAGCTGTCTCTGCAGGACAGGGTGTACCCTG(+ACTCAC)GATCCTTAGTAGCAGTTACAAAGCTTATAGC NGG 10 8 17 8 24 0 100 5 10
    240605003025_2rl4 AAAATGTACAAAGTCTGAATAAAGCATTTTGG(+ACTCAC)GATCCGCCATCCGGTCTCTGCATATACAGAT NGG 10 8 17 8 24 0 100 5 10
    240605003023_jvpn GTGTCATCTGTATATGCAGAGACCGGATGGCG(+ACTCAC)GATCCCAAAATGCTTTATTCAGACTTTGTAC NGG 10 8 17 8 24 0 100 5 10
    240605003022_4rca ACAGCACTGCAGGCAAGTCTTTGGTTCTGAAG(+ACTCAC)GATCCAAGATGGTACTGTTAGGCAGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240605003021_zwk7 TGCTAGCTGTGCTGCCTAACAGTACCATCTTG(+ACTCAC)GATCCTTCAGAACCAAAGACTTGCCTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605003019_a4m8 TTTTTTTTTTTTTTTTTTTTAGAATTTGCCTG(+ACTCAC)GTACCTTTGGAAAGCTTTACTTAAGCAATGG NGG 10 8 17 8 24 0 100 5 10
    240605003017_odga GGAGTCCATTGCTTAAGTAAAGCTTTCCAAAG(+ACTCAC)GTACCAGGCAAATTCTAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605003015_0y54 GGGGGAAGGATTGAGGAACCTGGAGGGAATAG(+ACTCAC)GTACCCCTTCAGAATACCAACAGAGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240605003013_reea AGGTTAGTTGCCTCTGTTGGTATTCTGAAGGG(+ACTCAC)GTACCTATTCCCTCCAGGTTCCTCAATCCTT NGG 10 8 17 8 24 0 100 5 10
    240605003012_hyvg ATTGCTTTACTGCATGTCATTGAATATGTAAG(+ACTCAC)GTACCTCAAAAATATTTGAAGAGTGGGAAAG NGG 10 8 17 8 24 0 100 5 10