Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002939_i67z ACATCTAGAGCTGTAAAGGGTTGGCTGCAATG(+ACTCAC)GTACCTCCCTTTGTAGCCCAGCAGACCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605002938_9nog GCAGATGTGTGTGGCATGTGGCCAGTTCACAG(+ACTCAC)GATCCTCCAGTTCTAAAACACACAGAGTACG NGG 10 8 17 8 24 0 100 5 10
    240605002936_y8mo CTGCCCGTACTCTGTGTGTTTTAGAACTGGAG(+ACTCAC)GATCCTGTGAACTGGCCACATGCCACACACA NGG 10 8 17 8 24 0 100 5 10
    240605002933_ljh0 AATGAGCTACCCAACTTCTACCACATTCCTGG(+ACTCAC)GTACCCAACTGTGTTGTTACTCTGAACCCAC NGG 10 8 17 8 24 0 100 5 10
    240605002932_uxja AAACTGTGGGTTCAGAGTAACAACACAGTTGG(+ACTCAC)GTACCCAGGAATGTGGTAGAAGTTGGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605002931_zmaq GAAGAAGCCCAAGAAAAAAGCAGGGTTATCTG(+ACTCAC)GTACCTATCCTGGGCAGAATCACAGGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605002929_2vws ATCAGTACAACCTGTGATTCTGCCCAGGATAG(+ACTCAC)GTACCAGATAACCCTGCTTTTTTCTTGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605002928_vxqw CACACGTTAAGATGCCTTTCTCGCCTCTGAAG(+ACTCAC)GATCCTGAGTCACTGCCCTCATTTACTATGA NGG 10 8 17 8 24 0 100 5 10
    240605002927_r225 GGGATTCATAGTAAATGAGGGCAGTGACTCAG(+ACTCAC)GATCCTTCAGAGGCGAGAAAGGCATCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605002925_gpkg TCAGTCTCAGCACTCAGAGACAGAGCTAGGTG(+ACTCAC)GATCCCTGAGTTCGAGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10