Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002835_f9rz TCCTGGGCCAGGGAGCCCAGGATGAGGGGTGG(+ACTCAC)GTACCTGGCAGCCATAGTTGCTCCTAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605002833_59ql GGGCCACGGCATTACAAGAGGGGCTGAAGTGG(+ACTCAC)GTACCAGGCTTTTCCTCATTTTTGGTACAAG NGG 10 8 17 8 24 0 100 5 10
    240605002832_gfc3 GATCACTTGTACCAAAAATGAGGAAAAGCCTG(+ACTCAC)GTACCCACTTCAGCCCCTCTTGTAATGCCGT NGG 10 8 17 8 24 0 100 5 10
    240605002830_24ut GGAAGGCTCCTGGCTCTAGGTCTGTGCCTCTG(+ACTCAC)GATCCACATTGGTCTTGGTCCATGTGGCACT NGG 10 8 17 8 24 0 100 5 10
    240605002828_g2g0 CAACCAGTGCCACATGGACCAAGACCAATGTG(+ACTCAC)GATCCAGAGGCACAGACCTAGAGCCAGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605002827_hczc CCAAGTCTATACAGCAAGACCCTTCACTCCTG(+ACTCAC)GATCCAGAGAGAACATAATGCTTTCAATCTG NGG 10 8 17 8 24 0 100 5 10
    240605002825_a4cc AGCAGCAGATTGAAAGCATTATGTTCTCTCTG(+ACTCAC)GATCCAGGAGTGAAGGGTCTTGCTGTATAGA NGG 10 8 17 8 24 0 100 5 10
    240605002824_e0tm ATTTGTGTTCTTCCTGGGTAATCTGGTCTTAG(+ACTCAC)GATCCCTCCAGGCCTCACTACTATCAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605002822_vrzr GGATAGATGCTGATAGTAGTGAGGCCTGGAGG(+ACTCAC)GATCCTAAGACCAGATTACCCAGGAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605002821_ou48 GGTGACCAATTATATGGTCAATTTTGGAGAAG(+ACTCAC)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10