Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002806_ps5u GTCTGTAGGTGGACAGTCCCCTTGGAGTTTAG(+ACTCAC)GATCCTGTGTCCCGTTCCCCACTTCGTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605002804_718s GGTGCTGTTGGCACCACCACCCGAAGACATGG(+ACTCAC)GTACCTGTACCAGACTTCATAAGCAGCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605002803_wweg TGGAGATGAGCTGCTTATGAAGTCTGGTACAG(+ACTCAC)GTACCCATGTCTTCGGGTGGTGGTGCCAACA NGG 10 8 17 8 24 0 100 5 10
    240605002801_qdmb CACCAGGCTAGGCTCATGGCCTTGAACTCAGG(+ACTCAC)GATCCACCTGCCTCTGCCTCCCTGTGATGCT NGG 10 8 17 8 24 0 100 5 10
    240605002800_xdvk ATCTCAGCATCACAGGGAGGCAGAGGCAGGTG(+ACTCAC)GATCCCTGAGTTCAAGGCCATGAGCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240605002758_g7qy GCCTGAAGAGGGCCTGGGGAACAGTGGAAATG(+ACTCAC)GTACCTTCATGGTGACTTCAGCAGGGTAGTC NGG 10 8 17 8 24 0 100 5 10
    240605002757_oyhx GTAATGACTACCCTGCTGAAGTCACCATGAAG(+ACTCAC)GTACCATTTCCACTGTTCCCCAGGCCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605002755_ky0r ATAGCTGTGGTATCAGGACTTGAGAGATGGAG(+ACTCAC)GATCCAAAAGTTTAGGGTCAGTCTGGGTATA NGG 10 8 17 8 24 0 100 5 10
    240605002754_ile6 TGCTGTATACCCAGACTGACCCTAAACTTTTG(+ACTCAC)GATCCTCCATCTCTCAAGTCCTGATACCACA NGG 10 8 17 8 24 0 100 5 10
    240605002752_lf4j TCCTGCCAGCCCTTGAGCCTCTGCTCTCCTGG(+ACTCAC)GTACCCTCCTGGAACACCATGCTAACCTTCC NGG 10 8 17 8 24 0 100 5 10