Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002655_g1hp AGTACGATGAGGAGCTCACCCCTGTCTAGGTG(+ACTCAC)GATCCCCAACTTCTCAGAGGCCCCAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240605002654_gh8g CCCACACCATGAGCAACAGGCAGCACCAACTG(+ACTCAC)GATCCAGGGTATTGATGTTAAGAACAAAACA NGG 10 8 17 8 24 0 100 5 10
    240605002651_1nz8 CACCTTGTTTTGTTCTTAACATCAATACCCTG(+ACTCAC)GATCCAGTTGGTGCTGCCTGTTGCTCATGGT NGG 10 8 17 8 24 0 100 5 10
    240605002649_81y8 ACAGGTAGGATTGCTGATCTGGGCACCACTGG(+ACTCAC)GATCCAAGCACTGTGTGAATCTGATCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605002648_39tv TGGTTTTCTAGATCAGATTCACACAGTGCTTG(+ACTCAC)GATCCCAGTGGTGCCCAGATCAGCAATCCTA NGG 10 8 17 8 24 0 100 5 10
    240605002647_crut AGCTGCTTCTTACTGCATAATGGCTGTTTCAG(+ACTCAC)GTACCATCACTTCACTGCTTGGCTTTTAGTC NGG 10 8 17 8 24 0 100 5 10
    240605002645_8c8a GCTTTGACTAAAAGCCAAGCAGTGAAGTGATG(+ACTCAC)GTACCTGAAACAGCCATTATGCAGTAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605002643_7aou CTCAAATGATTGAAGTTTCATAAACTGCCTAG(+ACTCAC)GATCCTGTCAAGGCCACTGGACTCTTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605002642_j8rb GGAAAAGAACAAGAGTCCAGTGGCCTTGACAG(+ACTCAC)GATCCTAGGCAGTTTATGAAACTTCAATCAT NGG 10 8 17 8 24 0 100 5 10
    240605002640_5950 GGCCTACTGGGGGGTCCCCAAACTCAGTCCTG(+ACTCAC)GTACCACCTCAACTGTGACAATCAGCAAAGC NGG 10 8 17 8 24 0 100 5 10