Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002625_bwx0 TTACAGACTGGGTGGTAAAGAGGGGCATCTTG(+ACTCAC)GTACCTGGGTCCCAGAGAGAGAAGTCAAACA NGG 10 8 17 8 24 0 100 5 10
    240605002622_4hof CATTTTGTTTGACTTCTCTCTCTGGGACCCAG(+ACTCAC)GTACCAAGATGCCCCTCTTTACCACCCAGTC NGG 10 8 17 8 24 0 100 5 10
    240605002621_b7fj TAGGGCTCACTTGTATATTAGTTTCTGAATTG(+ACTCAC)GATCCATAACTTTAAGATTAAACAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605002620_c00j TTATCTTTTCTCTGTTTAATCTTAAAGTTATG(+ACTCAC)GATCCAATTCAGAAACTAATATACAAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605002618_g2ai ACCCTTTCTCTGACACACAGAGAGAGCTGCAG(+ACTCAC)GTACCTAGCTGAGAACCAGGGCATGGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605002617_vn96 ATCCCCCTTCCCATGCCCTGGTTCTCAGCTAG(+ACTCAC)GTACCTGCAGCTCTCTCTGTGTGTCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605002616_huls ATTATTATTATTATTACTTGTGTGTGTGTGTG(+ACTCAC)GATCCCACATCCTCCAACAGCTCCAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605002614_m7l8 CTTCCAACCCCTGGAGCTGTTGGAGGATGTGG(+ACTCAC)GATCCACACACACACACAAGTAATAATAATA NGG 10 8 17 8 24 0 100 5 10
    240605002613_qvsq CTCAAAGCTTTGAGGAGTGAAGTTAGGAGTAG(+ACTCAC)GTACCAGTGACTACGAACTGAACATGCACTG NGG 10 8 17 8 24 0 100 5 10
    240605002611_kur3 GCTCACAGTGCATGTTCAGTTCGTAGTCACTG(+ACTCAC)GTACCTACTCCTAACTTCACTCCTCAAAGCT NGG 10 8 17 8 24 0 100 5 10