Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002521_y2iv CTCCATCCTTGCACTCGCCACTACCCTAGAAG(+ACTCAC)GATCCTTCTGCTGCCCTCTGCCTCCCATCCG NGG 10 8 17 8 24 0 100 5 10
    240605002520_sqv8 TGGGGCGGATGGGAGGCAGAGGGCAGCAGAAG(+ACTCAC)GATCCTTCTAGGGTAGTGGCGAGTGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605002519_wl1k TATTTTGGAATAAAATTTATTTTATGGCTTAG(+ACTCAC)GATCCATGGCCCCCTTCCCCTCCAGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605002516_xr81 TTTTTCCCTCACTGGAGGGGAAGGGGGCCATG(+ACTCAC)GATCCTAAGCCATAAAATAAATTTTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240605002515_jget TATCAGCCAGTGTGGCCCTCTATTTCCTTGTG(+ACTCAC)GTACCGAGTGTCCCTGAGGCTGGGGTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605002514_34nb GAAAAAGACCACCCCAGCCTCAGGGACACTCG(+ACTCAC)GTACCACAAGGAAATAGAGGGCCACACTGGC NGG 10 8 17 8 24 0 100 5 10
    240605002512_vv3d AGCATGCAGACATCCCTCTGTGGACTTCAGGG(+ACTCAC)GATCCACCTCTAAAGAGAGTCACTAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605002510_4boo CTGTTGGTCACTAGTGACTCTCTTTAGAGGTG(+ACTCAC)GATCCCCTGAAGTCCACAGAGGGATGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605002508_wufi GGGTTCCCACGTGTCTGGAAGCTCGAGTTCCG(+ACTCAC)GATCCATACTGACACCCAATAAGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605002507_jzij TACTGCTACACAGCTTATTGGGTGTCAGTATG(+ACTCAC)GATCCGGAACTCGAGCTTCCAGACACGTGGG NGG 10 8 17 8 24 0 100 5 10