Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002435_8d6w TGACAAGAGATAGTCTGGGCTCAGAAGCACAG(+ACTCAC)GTACCGGGAGACAGCAGAGACAGGAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605002433_hqzg AGCAGGATGCAAATGTGGAGGCAGTCCCAGAG(+ACTCAC)GATCCCAAAACTGCTGCCAAGTTACAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605002431_4me5 GGGTGTCTCCTGTAACTTGGCAGCAGTTTTGG(+ACTCAC)GATCCTCTGGGACTGCCTCCACATTTGCATC NGG 10 8 17 8 24 0 100 5 10
    240605002429_dcdi GGACAATCCTGACCCTCTTCCGCTGGCCTGTG(+ACTCAC)GATCCCTTGACAAGCGACTCTGTTCTGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605002428_q1td GACCCTGGACAGAACAGAGTCGCTTGTCAAGG(+ACTCAC)GATCCACAGGCCAGCGGAAGAGGGTCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605002424_dcvx CTGCTGGCATCACCACACTCAGCCAGCCCCTG(+ACTCAC)GATCCTTCTGACTTGGCTTTGCCATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605002423_zpgy AAAACAAAAAAATGGCAAAGCCAAGTCAGAAG(+ACTCAC)GATCCAGGGGCTGGCTGAGTGTGGTGATGCC NGG 10 8 17 8 24 0 100 5 10
    240605002421_ru4v AAGGAGGCAAGTCCTAACATGGGGGTGAGCTG(+ACTCAC)GATCCAGACACGGGTGGGTGCGTAGAGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605002420_gq5e CTCTCGGCACTCTACGCACCCACCCGTGTCTG(+ACTCAC)GATCCAGCTCACCCCCATGTTAGGACTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605002419_gctn GGGGCAGGCCAAAAGATGGATGATAAGAAGTG(+ACTCAC)GATCCACTTGAGAGTGGCTGAAGAACCAAGG NGG 10 8 17 8 24 0 100 5 10