Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002505_y8j3 AGACTCGGCCAGAGACAGACGTTAGAGGACGG(+ACTCAC)GATCCAGTCCCTCTCCCACAGCAGGCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605002503_y6sy TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+ACTCAC)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10
    240605002502_s7gh GCAGAACCTAGGGCTTTGACCATGGCTTAGTG(+ACTCAC)GTACCATGCGAAGACACTTAGTGTGACAAGT NGG 10 8 17 8 24 0 100 5 10
    240605002501_a7gz CTCAGACTTGTCACACTAAGTGTCTTCGCATG(+ACTCAC)GTACCACTAAGCCATGGTCAAAGCCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240605002459_bohg ATCTGCCCATTTCTGTCCCCTGGGGTACTGGG(+ACTCAC)GATCCAAACATAGTCCTCATGTCTGTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605002458_ju45 TGCTTACTGAACAGACATGAGGACTATGTTTG(+ACTCAC)GATCCCCAGTACCCCAGGGGACAGAAATGGG NGG 10 8 17 8 24 0 100 5 10
    240605002456_kufq AGTGTGCTTGCCTAGCATGTACAAAGCTCTGG(+ACTCAC)GATCCACCCCCAGCACCACTTAAGCCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240605002454_czk8 CGCCACGCCTGGCTTAAGTGGTGCTGGGGGTG(+ACTCAC)GATCCCAGAGCTTTGTACATGCTAGGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605002453_ukh2 CTTTGCCAAGGCCCCCCGCTGTCCTCTACTAG(+ACTCAC)GATCCAACGGAAGCCTCTGCTCCCCCCGCCT NGG 10 8 17 8 24 0 100 5 10
    240605002451_r2nc GAGAGAGGCGGGGGGAGCAGAGGCTTCCGTTG(+ACTCAC)GATCCTAGTAGAGGACAGCGGGGGGCCTTGG NGG 10 8 17 8 24 0 100 5 10