Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002536_bb5e CACTCGCTGGTCCCAACCTCCTCATATGTGTG(+ACTCAC)GATCCTCAAGGAAGGCCATGATGATCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605002535_003u CCCAAGCCCTGATCATCATGGCCTTCCTTGAG(+ACTCAC)GATCCACACATATGAGGAGGTTGGGACCAGC NGG 10 8 17 8 24 0 100 5 10
    240605002534_pvxd TGCAATTACTGCAATCCCTCTGAAAGTGCTTG(+ACTCAC)GATCCGCCGGCAAATATTAAAAGGAGATTTC NGG 10 8 17 8 24 0 100 5 10
    240605002532_ofs6 TTGTGGAAATCTCCTTTTAATATTTGCCGGCG(+ACTCAC)GATCCAAGCACTTTCAGAGGGATTGCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240605002530_ohjg TCACACAGCACTTACAATGTGGCCACACCAAG(+ACTCAC)GTACCATGCCAGTCTATATGAAAACAACAGA NGG 10 8 17 8 24 0 100 5 10
    240605002528_auyr AGCAGTCTGTTGTTTTCATATAGACTGGCATG(+ACTCAC)GTACCTTGGTGTGGCCACATTGTAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605002526_yx17 GTGGGCACAGCTAAGGTCCATTTTAACTCTGG(+ACTCAC)GATCCATCTCAGCATACCTTTCAACAATTAT NGG 10 8 17 8 24 0 100 5 10
    240605002525_smvs GTTACATAATTGTTGAAAGGTATGCTGAGATG(+ACTCAC)GATCCCAGAGTTAAAATGGACCTTAGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605002524_v73l TCAATATTTAAATCATTCAGGATTATTATAAG(+ACTCAC)GATCCAGTGAAATAGAGCAGACAGAAGTAAA NGG 10 8 17 8 24 0 100 5 10
    240605002522_swrf CATTTTTTACTTCTGTCTGCTCTATTTCACTG(+ACTCAC)GATCCTTATAATAATCCTGAATGATTTAAAT NGG 10 8 17 8 24 0 100 5 10