Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002450_wbn9 TGAAAGGACTCTTTTCTGCCCAAGGGACACAG(+ACTCAC)GATCCGTCAGTTCCACTCCCTGCATGCACCA NGG 10 8 17 8 24 0 100 5 10
    240605002448_23oo GGTCGTGGTGCATGCAGGGAGTGGAACTGACG(+ACTCAC)GATCCTGTGTCCCTTGGGCAGAAAAGAGTCC NGG 10 8 17 8 24 0 100 5 10
    240605002446_9hqu TTGGCCACTTGACTTTGGTCCAGTGATCCCGG(+ACTCAC)GTACCGGTAACGGGCATAAGTTTCAGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605002444_cgtt GACCCAGGAGCTGAAACTTATGCCCGTTACCG(+ACTCAC)GTACCCGGGATCACTGGACCAAAGTCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240605002443_jnmg GGGGTATGTGGGAGCCGGTACACACGGTGCTG(+ACTCAC)GATCCAGAGCTGGGGTGGGGTGCATGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605002441_x3p5 ACCCCAGGGACATGCACCCCACCCCAGCTCTG(+ACTCAC)GATCCAGCACCGTGTGTACCGGCTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605002440_4jcu GGGGTATGTGGGAGCCGGTACACACGGTGCTG(+ACTCAC)GATCCAGAGCTGGGGTGGGGTGCATGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605002439_6v3p ACCCCAGGGACATGCACCCCACCCCAGCTCTG(+ACTCAC)GATCCAGCACCGTGTGTACCGGCTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605002437_d7gf GAACGCCTACTTCCTGTCTCTGCTGTCTCCCG(+ACTCAC)GTACCTGTGCTTCTGAGCCCAGACTATCTCT NGG 10 8 17 8 24 0 100 5 10
    240605002435_8d6w TGACAAGAGATAGTCTGGGCTCAGAAGCACAG(+ACTCAC)GTACCGGGAGACAGCAGAGACAGGAAGTAGG NGG 10 8 17 8 24 0 100 5 10