Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002552_mjfc CCTGCGTCTCCCAGCATGGTAGGTAGCCTCAG(+ACTCAC)GATCCTAGAAAGATTATGGCCAGTGTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605002551_1qj1 GTCAGAACAGACACTGGCCATAATCTTTCTAG(+ACTCAC)GATCCTGAGGCTACCTACCATGCTGGGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605002549_42qm CAAGCAACTGGTAGCTCTCACAATTCTGTTGG(+ACTCAC)GATCCTTTGTGCAATATTGTAGCCATCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605002548_filr CCAGGGGAAGATGGCTACAATATTGCACAAAG(+ACTCAC)GATCCCAACAGAATTGTGAGAGCTACCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605002547_joam TCTAGGGGCAAGCCAGCTCAAGCAGCCTGAAG(+ACTCAC)GATCCCGGATTGGTGGCTGTTGGAATGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605002544_ochf TCATTTCTTCATTCCAACAGCCACCAATCCGG(+ACTCAC)GATCCTTCAGGCTGCTTGAGCTGGCTTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605002543_c7nz GCATGTATCAATACACATTACATTCATGCTTG(+ACTCAC)GTACCTGTGGAGGTCAGAAGGGGGCATCCGA NGG 10 8 17 8 24 0 100 5 10
    240605002541_8ng6 GGGGATCGGATGCCCCCTTCTGACCTCCACAG(+ACTCAC)GTACCAAGCATGAATGTAATGTGTATTGATA NGG 10 8 17 8 24 0 100 5 10
    240605002540_o54b TCAATGGTGATTAGAGAAGCAAGCATTATATG(+ACTCAC)GATCCCAATCAGATAGAGAAATGCAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605002538_q6za TTTCTTTTCTTTGCATTTCTCTATCTGATTGG(+ACTCAC)GATCCATATAATGCTTGCTTCTCTAATCACC NGG 10 8 17 8 24 0 100 5 10