Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001854_cwwc CTATAAAATGAGGTATGTGAGGAGGACTGTGG(+ACTCAC)GTACCTGGGCACAAGATTTCTGAAACCGGTA NGG 10 8 17 8 24 0 100 5 10
    240605001852_hwmc AAGCTCATGCTTGCAAGGCCAGGAAAGTCGTG(+ACTCAC)GATCCCTGACAATGAGGGCAGTGGTTCATTG NGG 10 8 17 8 24 0 100 5 10
    240605001851_wff3 AGCAACAATGAACCACTGCCCTCATTGTCAGG(+ACTCAC)GATCCACGACTTTCCTGGCCTTGCAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605001849_fzqi AATAGAGCCCTGAAGTGCTGACAGGGCTGAGG(+ACTCAC)GATCCTTGGCTGCCATATCCCACAGAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605001847_a7ca GAAGAGTCTCTCTGTGGGATATGGCAGCCAAG(+ACTCAC)GATCCCTCAGCCCTGTCAGCACTTCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605001845_ja37 TAGATAGGCTCACAGGAGAACTTCATCTAGAG(+ACTCAC)GATCCCTCTGTGAAGACTCTCCCTCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605001844_uk6x GAAGTGACCTGGAGGGAGAGTCTTCACAGAGG(+ACTCAC)GATCCTCTAGATGAAGTTCTCCTGTGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605001842_itom TCCTTCAGAATTTTCTTGGTTGTTGATTTGGG(+ACTCAC)GATCCTCAATCTCTCAACTTAGTTCCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605001840_k6yj TTGACATTAGGGAACTAAGTTGAGAGATTGAG(+ACTCAC)GATCCCCAAATCAACAACCAAGAAAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240605001839_k3x0 TTGCTAGAGGAAAAATAGACAGAGAATCTAAG(+ACTCAC)GTACCCCTGTCCCCAGAGCATGGTCATTTCC NGG 10 8 17 8 24 0 100 5 10