Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001837_xr6f CCCGAGGAAATGACCATGCTCTGGGGACAGGG(+ACTCAC)GTACCTTAGATTCTCTGTCTATTTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605001835_mobf ACATCCACCATATGCCTGCTCCCCTGTCGCCG(+ACTCAC)GTACCAAGTGAGATGTCTGTGTGGCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605001834_3o4u TGCAAAAACAAGCCACACAGACATCTCACTTG(+ACTCAC)GTACCGGCGACAGGGGAGCAGGCATATGGTG NGG 10 8 17 8 24 0 100 5 10
    240605001833_5dxx TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+ACTCAC)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605001832_3lw5 TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+ACTCAC)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605001830_wfpe TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+ACTCAC)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605001829_ykz5 TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+ACTCAC)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605001828_copg TTTAGCCTTTGTGGGAAAGAGTAAGGCATGTG(+ACTCAC)GATCCGACCAGGTGGTAAGGTTGTAAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605001826_zyqb GACAAGTTCCTTACAACCTTACCACCTGGTCG(+ACTCAC)GATCCACATGCCTTACTCTTTCCCACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605001825_xuun CTATAAAATGAGGTATGTGAGGAGGACTGTGG(+ACTCAC)GTACCTGGGCACAAGATTTCTGAAACCGGTA NGG 10 8 17 8 24 0 100 5 10