Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001750_4b9l AGCAACAATGAACCACTGCCTTCATTGTCAGG(+ACTCAC)GATCCACGACTTTCCTGGCCTTGCAAGCACG NGG 10 8 17 8 24 0 100 5 10
    240605001748_xlfp AATAGAGCCCTGAAGTGCTGACAGGGCTGAGG(+ACTCAC)GATCCTTGGCTGCCATGTCCCACACAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605001746_yo3x GAAGAGTCTCTGTGTGGGACATGGCAGCCAAG(+ACTCAC)GATCCCTCAGCCCTGTCAGCACTTCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605001745_op4c GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+ACTCAC)GATCCCTGCATATGGCAGTCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605001743_1yg9 GGATGGACCATCTAGTGACTGCCATATGCAGG(+ACTCAC)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605001741_ilqn TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ACTCAC)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605001739_tvxm TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605001737_h1ly AGGAGTTGATGCTGCAGATAGACTCAGACTGG(+ACTCAC)GTACCTTGCACACCACACCCATGAAACGAGA NGG 10 8 17 8 24 0 100 5 10
    240605001736_7dph TCAAGTCTCGTTTCATGGGTGTGGTGTGCAAG(+ACTCAC)GTACCCAGTCTGAGTCTATCTGCAGCATCAA NGG 10 8 17 8 24 0 100 5 10
    240605001734_hij6 ACAGAAGAAACGCCAGCGTGAAGGGGCCACGG(+ACTCAC)GATCCTTAACTACATACAATAGAAAAGTGTT NGG 10 8 17 8 24 0 100 5 10