Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001643_d8se CCATTCTGCCCATCTGTTGGTGCTGGGCGCAG(+ACTCAC)GTACCAGGCCAGGATTGCCTGGTCTGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605001641_8g27 CACAGCACCCAGCCTCACTACCCCAGCATGGG(+ACTCAC)GTACCCAGCTGGATACTCCCAAAATGTCACA NGG 10 8 17 8 24 0 100 5 10
    240605001640_f8g9 TGGGATGTGACATTTTGGGAGTATCCAGCTGG(+ACTCAC)GTACCCCATGCTGGGGTAGTGAGGCTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605001636_9d04 ATTCTAGCCCACACCTGGGACCACCACCCCAG(+ACTCAC)GTACCTGGGGGACAGTGAGGCAAGACACAGA NGG 10 8 17 8 24 0 100 5 10
    240605001633_ttll GGACCTCTGTGTCTTGCCTCACTGTCCCCCAG(+ACTCAC)GTACCTGGGGTGGTGGTCCCAGGTGTGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605001630_dhxq GGGCTGTTCCATTTCTTTACTGAGACACCCAG(+ACTCAC)GATCCATTTGGCAAGTGACCCAGAGCAACAG NGG 10 8 17 8 24 0 100 5 10
    240605001629_xdzg GGACACTGTTGCTCTGGGTCACTTGCCAAATG(+ACTCAC)GATCCTGGGTGTCTCAGTAAAGAAATGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605001628_8oi5 CTGTTGTGTTTTTTTTTTCCTTTTTGTTATTG(+ACTCAC)GTACCAGGCAATTAAAAAAAAAAAAAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605001626_nvzj CCTCTACTTTTTTTTTTTTTTTTAATTGCCTG(+ACTCAC)GTACCAATAACAAAAAGGAAAAAAAAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605001623_5yy7 CCCCTGGGCCCATTCTGCTGGCCCCAAGGGGG(+ACTCAC)GATCCTGAGACATCTGGGGGGGGGGGGAGGG NGG 10 8 17 8 24 0 100 5 10