Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001528_0tf2 TGAGACCCATCCCAAGTGGCCAAAACAAGCTG(+ACTCAC)GATCCTTACAGCATCCCCATGCCCAATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605001525_iuvc TTAGCTTACTCACCCTTTGTCCCCTCCCCCAG(+ACTCAC)GTACCATGTGCCGAGGCCTCAGACACAGCGT NGG 10 8 17 8 24 0 100 5 10
    240605001524_7630 GGACTACGCTGTGTCTGAGGCCTCGGCACATG(+ACTCAC)GTACCTGGGGGAGGGGACAAAGGGTGAGTAA NGG 10 8 17 8 24 0 100 5 10
    240605001522_ivm3 CGGCGCTGTCCAGTCTAAACCCCTGGAGGTTG(+ACTCAC)GATCCTCAGCAGGGCTCCTGTTCTGGCGTCC NGG 10 8 17 8 24 0 100 5 10
    240605001521_cbyl TCATGGGACGCCAGAACAGGAGCCCTGCTGAG(+ACTCAC)GATCCAACCTCCAGGGGTTTAGACTGGACAG NGG 10 8 17 8 24 0 100 5 10
    240605001519_ipyh CAGAGATGCCTGGCTGTCAGGACAGAAGGCCG(+ACTCAC)GTACCCCAAGGTCCTGCTCTCAGGCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605001517_yokp CTTCTCTTCCAGCCTGAGAGCAGGACCTTGGG(+ACTCAC)GTACCGGCCTTCTGTCCTGACAGCCAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605001516_ovru CAGTTCTCCCTTAAGCCAGGCTAGATCACTGG(+ACTCAC)GTACCCAAGCCCCCTGCCACCCCTTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605001514_0k58 CCTGAGCTGCCAAGGGGTGGCAGGGGGCTTGG(+ACTCAC)GTACCCAGTGATCTAGCCTGGCTTAAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605001512_u8nq GTGGCTGGGAGAAAGATGGCCCTTGCATGCTG(+ACTCAC)GTACCACAGCCCAGACATCTCTGCTGCTCTA NGG 10 8 17 8 24 0 100 5 10