Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001437_gw3w TGAAGTTCTAGGGAGGAGGATGGGCACCTGTG(+ACTCAC)GATCCCCTCCCATAGGTAGCAGAGCTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605001435_jn1l CTGCAGGTGCTGGTCCTGCAAAGTCCAAGCGG(+ACTCAC)GTACCCCTTCCTGGGTGATGACCCTGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605001433_0g7m GCATTCTGCACAGGGTCATCACCCAGGAAGGG(+ACTCAC)GTACCCGCTTGGACTTTGCAGGACCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240605001432_5vsx GTTGTGGGGTTCGTAGCAGTCCCAGGATCCTG(+ACTCAC)GTACCTAAGGCAGCTGGGACCCAGAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605001430_pner GGAAACTCTGTTCTGGGTCCCAGCTGCCTTAG(+ACTCAC)GTACCAGGATCCTGGGACTGCTACGAACCCC NGG 10 8 17 8 24 0 100 5 10
    240605001427_vug0 ACATAGAGTTGTGGGGTTCGTAGCAGTCCCAG(+ACTCAC)GATCCTGGTACCTAAGGCAGCTGGGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605001426_4jsc CTGTTCTGGGTCCCAGCTGCCTTAGGTACCAG(+ACTCAC)GATCCTGGGACTGCTACGAACCCCACAACTC NGG 10 8 17 8 24 0 100 5 10
    240605001424_127i TGAAGATACACCTAATCCTCAAAGAACAGGAG(+ACTCAC)GATCCAGGGAGTGTGCAGGTTTGTTGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605001422_xdut TCCTCCACCCCAACAAACCTGCACACTCCCTG(+ACTCAC)GATCCTCCTGTTCTTTGAGGATTAGGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605001420_cue5 CGGAGCAGTGTCAGAGGCCCAGGTGGATGCTG(+ACTCAC)GTACCAGCAGATTAAACAGCAGCCATCCTGC NGG 10 8 17 8 24 0 100 5 10