Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001328_bba7 CACCAGGGTTGAGGAGCACAGAGGGTCACGTG(+ACTCAC)GTACCAGATATCAATTATATGTATATGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605001326_iqr9 TGCAGCCATAGCAGGGCAACAGCAAGGCCTGG(+ACTCAC)GATCCCTTTGTCCAGACCCTGGGCAAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605001324_z9hi GCTTCTGGCCTTGCCCAGGGTCTGGACAAAGG(+ACTCAC)GATCCCAGGCCTTGCTGTTGCCCTGCTATGG NGG 10 8 17 8 24 0 100 5 10
    240605001323_nfso CAACTCCCACAGGCACTTGAGGTGACACAGAG(+ACTCAC)GTACCTGATACTCGTAGGAGTCAGATGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605001322_8ua7 GGACCTGAGCATCTGACTCCTACGAGTATCAG(+ACTCAC)GTACCTCTGTGTCACCTCAAGTGCCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605001320_ileb TTTTTCAGTCCCAGGTTGACCTCACAGCAAGG(+ACTCAC)GATCCAGGTCCTGGAGTGCTTAGGCATACAG NGG 10 8 17 8 24 0 100 5 10
    240605001319_0ujb TGATGCTGTATGCCTAAGCACTCCAGGACCTG(+ACTCAC)GATCCCTTGCTGTGAGGTCAACCTGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605001317_ga7g CCTCATGGGCTCTCCGTGGTGAAGAACAAAGG(+ACTCAC)GTACCCCAATGGCCTCTCACCATCACTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605001315_kj6o CTGGAGGACAGTGATGGTGAGAGGCCATTGGG(+ACTCAC)GTACCCTTTGTTCTTCACCACGGAGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605001314_bzft CCACTTCTCCTGGAGTCCCAATCAGCACACAG(+ACTCAC)GATCCTCATGTGGGTGTAGGCTCAGCTGTCC NGG 10 8 17 8 24 0 100 5 10