Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001245_6qcd TTAGTAACTAAAGCCTAACTCCAGCTCCAGGG(+ACTCAC)GATCCGGCACCCTCTTCTGTCCCCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240605001243_p5te AGCAGAGCCCTGGCCCTATTAGCAGCACAGTG(+ACTCAC)GTACCCGAGGGTCTCTGTCAACAAGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605001241_nkru TCATTTCCTGTCTTGTTGACAGAGACCCTCGG(+ACTCAC)GTACCACTGTGCTGCTAATAGGGCCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605001240_v100 CACACACAGCACCCATGCTAGCACGCACAGAG(+ACTCAC)GATCCCGTTGATCACTCCAGAAGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605001238_40je ACCCAGGTCACCTCTTCTGGAGTGATCAACGG(+ACTCAC)GATCCTCTGTGCGTGCTAGCATGGGTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605001236_7d78 AATTAAATGTTGTCCTTATAAGAGTTGCCTTG(+ACTCAC)GTACCAGGAGAGCCAGGGCTATACAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605001234_vj9g CAGGGTTTCTCTGTATAGCCCTGGCTCTCCTG(+ACTCAC)GTACCAAGGCAACTCTTATAAGGACAACATT NGG 10 8 17 8 24 0 100 5 10
    240605001232_vmun TTCAAAACAATGAAATAAAACCTAAGCCGTAG(+ACTCAC)GTACCTAAAATGAGGTTATTTCTCAACCCCT NGG 10 8 17 8 24 0 100 5 10
    240605001231_tlr3 GGATAAGGGGTTGAGAAATAACCTCATTTTAG(+ACTCAC)GTACCTACGGCTTAGGTTTTATTTCATTGTT NGG 10 8 17 8 24 0 100 5 10
    240605001230_82bb CAAGAGTCAGTGAAAGAGTCCCGAGAGCGCTG(+ACTCAC)GATCCCCATCCTTGTCCAGGGTGAAGTCACT NGG 10 8 17 8 24 0 100 5 10