Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001726_3nad GAGCAGCAGTCTCTGGTGTTCCCATCAGGGAG(+ACTCAC)GTACCTGCACGAGCGGGAGAAGCTTGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605001725_k2bs GGAAGAAAGACTGAGACCATCTGCTTTGGATG(+ACTCAC)GATCCATTAGCAAGCCTGGTCTGACTACACA NGG 10 8 17 8 24 0 100 5 10
    240605001723_mtxw GCCCCTGTGTAGTCAGACCAGGCTTGCTAATG(+ACTCAC)GATCCATCCAAAGCAGATGGTCTCAGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605001721_xn5f CTTTGTGGTAAGTCTTTGCTTCAGACTGTTGG(+ACTCAC)GTACCCAGAACGAGAGCTATAAAAATGCATT NGG 10 8 17 8 24 0 100 5 10
    240605001720_sxoe TGAACAATGCATTTTTATAGCTCTCGTTCTGG(+ACTCAC)GTACCCAACAGTCTGAAGCAAAGACTTACCA NGG 10 8 17 8 24 0 100 5 10
    240605001718_abei CTTTATTTGGATAGCAGCGCTTCAGTTCCTAG(+ACTCAC)GATCCTTTTATAAATGGTAAAACCCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240605001717_k53d TTGTAAATCCAGGGTTTTACCATTTATAAAAG(+ACTCAC)GATCCTAGGAACTGAAGCGCTGCTATCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605001715_o98m CTGACAAAGAGGTGGGCCAGGATGGGACATGG(+ACTCAC)GTACCAGGAGTGCACAGGCTTGGGCAATTTG NGG 10 8 17 8 24 0 100 5 10
    240605001713_5ab8 ATACACAAATTGCCCAAGCCTGTGCACTCCTG(+ACTCAC)GTACCCATGTCCCATCCTGGCCCACCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605001711_7end TATTAAAGGCGAGGGGAGAGAAATTGGGGAAG(+ACTCAC)GTACCTGCGTTTTATAAATAGCCTCAGGCAG NGG 10 8 17 8 24 0 100 5 10