Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001228_7vy4 TCCCAAGTGACTTCACCCTGGACAAGGATGGG(+ACTCAC)GATCCAGCGCTCTCGGGACTCTTTCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240605001226_8p94 TCTGTCCTTAGACACACCAGAAAAGGGCATCG(+ACTCAC)GATCCCATTACAGATAGCTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605001225_qs8k ACCACATGGTGGCTCACAGCTATCTGTAATGG(+ACTCAC)GATCCGATGCCCTTTTCTGGTGTGTCTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605001223_lc74 CAGCCTCGAGAAATTTCTCCTATTATATTTGG(+ACTCAC)GATCCAGCATGTCCCAAGACAGGGACGACTC NGG 10 8 17 8 24 0 100 5 10
    240605001222_hr2o TTATTGAGTCGTCCCTGTCTTGGGACATGCTG(+ACTCAC)GATCCCAAATATAATAGGAGAAATTTCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605001221_mx2n TTCTGGGGGCAGAGTTCTGGTTACTTTTCACG(+ACTCAC)GATCCGCAGAAGAACTGCCTAGGTGCCTAGG NGG 10 8 17 8 24 0 100 5 10
    240605001220_wuij ACACCCCTAGGCACCTAGGCAGTTCTTCTGCG(+ACTCAC)GATCCGTGAAAAGTAACCAGAACTCTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605001218_6e5e GGAACCAGGGATTCTGTAGCAGACATGGGCTG(+ACTCAC)GATCCATACACGGTCCCTGTGAGTTCATTTC NGG 10 8 17 8 24 0 100 5 10
    240605001217_m4s5 ATTGAGAAATGAACTCACAGGGACCGTGTATG(+ACTCAC)GATCCAGCCCATGTCTGCTACAGAATCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605001215_69n3 AACTGAATGGCTAATTTATTGAGAACCTATTG(+ACTCAC)GATCCACTCACAGGATTGAAGGACGCCCTCA NGG 10 8 17 8 24 0 100 5 10