Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001126_xwal CACATGGTGGCTCACAACCATCTGTAATGGGG(+ACTCAC)GATCCGATGCCTTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605001125_b7g0 GTCAGGGTGAAGGAGGTCACCTAAGGACCTTG(+ACTCAC)GATCCCAGTTATCACATGCAGGTGGCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605001123_rrm8 AGAAGACAAAGCCACCTGCATGTGATAACTGG(+ACTCAC)GATCCAAGGTCCTTAGGTGACCTCCTTCACC NGG 10 8 17 8 24 0 100 5 10
    240605001121_fddi AGACCTTAAGGGTCCAGGCCCACCCCTGCCAG(+ACTCAC)GATCCCTGGTCTGCTTTCTCCAGGACACACT NGG 10 8 17 8 24 0 100 5 10
    240605001119_pb7c GTCCCAGTGTGTCCTGGAGAAAGCAGACCAGG(+ACTCAC)GATCCTGGCAGGGGTGGGCCTGGACCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605001118_1ei3 TCACTCATCAGGAGCAGAATGAGGGACAAAAG(+ACTCAC)GTACCCAGGAATCGGGACCCAGGAATTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605001115_1u8l GTTAGAGACAATTCCTGGGTCCCGATTCCTGG(+ACTCAC)GTACCTTTTGTCCCTCATTCTGCTCCTGATG NGG 10 8 17 8 24 0 100 5 10
    240605001112_13x7 AGTCTTTTTTACACTCTGCCTGTGGTCCCAGG(+ACTCAC)GATCCTGGCTCAGAGGACTCAAGCGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605001111_oy4x CCTGTCCCCTGCGCTTGAGTCCTCTGAGCCAG(+ACTCAC)GATCCCTGGGACCACAGGCAGAGTGTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605001109_33jd CTTGTGGGGCTTTTAGTTTGAAGACCCTGAAG(+ACTCAC)GATCCCACTGCATAGTGAGTCTGTCCTGGTT NGG 10 8 17 8 24 0 100 5 10