Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001035_mrey CTTGCTCCAGCCCCCTCCCTTGGTTTCTGTTG(+ACTCAC)GTACCCATTTGGCTTTTATCTGCTTTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605001033_swb4 ACTGGAGCCAGGGTCCTCCGCACTGACTTCCG(+ACTCAC)GATCCATTTGCTAAGTTGCCTGCTGTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605001032_q6uh GACCTGGACAACAGCAGGCAACTTAGCAAATG(+ACTCAC)GATCCGGAAGTCAGTGCGGAGGACCCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605001030_zj0i AATTCTAATTCAAGAAATACAGCTTCTCAGGG(+ACTCAC)GATCCTTTAGGATCTGTGTTCTTATTGCACA NGG 10 8 17 8 24 0 100 5 10
    240605001029_xplq AGCCATGTGCAATAAGAACACAGATCCTAAAG(+ACTCAC)GATCCCCTGAGAAGCTGTATTTCTTGAATTA NGG 10 8 17 8 24 0 100 5 10
    240605001027_m5hk TTCATTTTAAGCTGGACCCGGTCTCTGCAAAG(+ACTCAC)GTACCAGAATCTCTCGGCCCTGCACCCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605001026_ftve TCAGAAAAGGGGTGCAGGGCCGAGAGATTCTG(+ACTCAC)GTACCTTTGCAGAGACCGGGTCCAGCTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605001025_ui2a TCTTAGGGAATAAAGGGATCTTAAATGCTGAG(+ACTCAC)GATCCAGCATCATGGAATCCAAGTGCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605001024_hapd TAAGCCCTTTGCACTTGGATTCCATGATGCTG(+ACTCAC)GATCCTCAGCATTTAAGATCCCTTTATTCCC NGG 10 8 17 8 24 0 100 5 10
    240605001021_22zq GTGGCTCACAACTGTCTGTAACTCTGTCCAGG(+ACTCAC)GATCCAATGGCCACTTCTGAACTCCCCAGGC NGG 10 8 17 8 24 0 100 5 10