Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000949_v7yd TTTCTGGGTAGGTATACATTAGTGCATCAGAG(+ACTCAC)GTACCCTCTGAGAGATGCTTGTCACTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605000948_w8bs CATGAGCAAACTTTTGAAACATCTTCAAGAAG(+ACTCAC)GTACCAAGGGATTGCAGTGCATAATTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605000946_eluo TTACAGCCAAAATTATGCACTGCAATCCCTTG(+ACTCAC)GTACCTTCTTGAAGATGTTTCAAAAGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240605000944_f1i6 TCTGACCCACATCAAGCCTCAGACGACAGATG(+ACTCAC)GTACCTGCCTGTTAAAGCATAACAGGGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605000943_dw2a CACCCATCACCCTGTTATGCTTTAACAGGCAG(+ACTCAC)GTACCATCTGTCGTCTGAGGCTTGATGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605000941_fwb8 ACTCCCCACTGGCATAAGGTGCCGGTCTGTGG(+ACTCAC)GTACCATCCGAGCTCCTGAGCAATGCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605000940_elmx TTGGGACTGAGCATTGCTCAGGAGCTCGGATG(+ACTCAC)GTACCCACAGACCGGCACCTTATGCCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605000938_fl29 TACCGCTTTCTCTGAGCACTCTGGGTGGCCAG(+ACTCAC)GTACCATCAACGTTTTGATCCAATACCTATG NGG 10 8 17 8 24 0 100 5 10
    240605000937_1vmp GCTGTCATAGGTATTGGATCAAAACGTTGATG(+ACTCAC)GTACCTGGCCACCCAGAGTGCTCAGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605000935_1uvu AACTTGGCAATAGCCAAGTCTAGAAGATGCTG(+ACTCAC)GTACCAAATAGCCTAATTCTTGTGATTGGAG NGG 10 8 17 8 24 0 100 5 10