Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001003_hoed TGATCTGTTGCAGATCAAATTATGAGGGAGTG(+ACTCAC)GATCCATCCCTATAAATTATTTGTAAGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605001001_gwd6 AATTGCTTAGGTTAGTTCATCTGGAACACAGG(+ACTCAC)GTACCACTATGAAGCTCAATTTCACTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605001000_b0xc TATCTGCTCAAGTGAAATTGAGCTTCATAGTG(+ACTCAC)GTACCCTGTGTTCCAGATGAACTAACCTAAG NGG 10 8 17 8 24 0 100 5 10
    240605000959_6uc0 AACATTTATACAAACCCTGTAACTCCATCAAG(+ACTCAC)GATCCATTTGAATGGTATTGAAGTCAGCCAA NGG 10 8 17 8 24 0 100 5 10
    240605000958_f0kd GTAGCTTGGCTGACTTCAATACCATTCAAATG(+ACTCAC)GATCCTTGATGGAGTTACAGGGTTTGTATAA NGG 10 8 17 8 24 0 100 5 10
    240605000956_ez65 TTAGAGAAGGTGGTCCCTCAGGATGCAGCTGG(+ACTCAC)GATCCACGCAGGCCCCAGGACACTAGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605000955_plph GTAGGGCAGCCTAGTGTCCTGGGGCCTGCGTG(+ACTCAC)GATCCCAGCTGCATCCTGAGGGACCACCTTC NGG 10 8 17 8 24 0 100 5 10
    240605000953_46qm GTTCTGTAAACAGTAAGTGAGGTCGTCTCTAG(+ACTCAC)GATCCTGAAGAGTCACACATGCACGCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605000952_38uf AAATGTGCAAGCGTGCATGTGTGACTCTTCAG(+ACTCAC)GATCCTAGAGACGACCTCACTTACTGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605000951_pk2k GAATTCCTTTAGTGACAAGCATCTCTCAGAGG(+ACTCAC)GTACCTCTGATGCACTAATGTATACCTACCC NGG 10 8 17 8 24 0 100 5 10