Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000906_jbdt GGGATCTGATGCCCTCTTCTGATACCCTACAG(+ACTCAC)GTACCACCCACGTGGTCTGCTTACATACATA NGG 10 8 17 8 24 0 100 5 10
    240605000905_vjbz ACCCCCAGGAGAGAGTGGTGGAGAGAGTGGAG(+ACTCAC)GATCCCGCAGGTCTTTGCCAATTAAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605000903_5sri TACCCCAAAGTTTAATTGGCAAAGACCTGCGG(+ACTCAC)GATCCTCCACTCTCTCCACCACTCTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605000900_egjm AGAAAACAGCCTGGAAGGGTAAGTACCCCCGG(+ACTCAC)GTACCATACACTTCTGAAATCCCGAGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605000858_u9fy GCCCCAAAGGCTCGGGATTTCAGAAGTGTATG(+ACTCAC)GTACCCGGGGGTACTTACCCTTCCAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605000857_qu3m CTCAGCCTTCCACGTGCTGTGATTATAGGCAG(+ACTCAC)GTACCACCATATACAGCATATTGTATGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605000855_f7u2 TTATAGACACATACAATATGCTGTATATGGTG(+ACTCAC)GTACCTGCCTATAATCACAGCACGTGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605000854_r0kq AGGTCCTCCTGCTTCAGCTTCTTACACAGAGG(+ACTCAC)GATCCCAGAGTGTGCCACCACCGCCAGCGTC NGG 10 8 17 8 24 0 100 5 10
    240605000852_jkp8 GCGGTGACGCTGGCGGTGGTGGCACACTCTGG(+ACTCAC)GATCCCTCTGTGTAAGAAGCTGAAGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605000850_l3lj CTTCCAGCAGGAGACCCCGCCCTCACTGGGTG(+ACTCAC)GATCCTTCTTTAGTATCACGTGCTCACACTG NGG 10 8 17 8 24 0 100 5 10