Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000848_o4vj CAAGCCAGTGTGAGCACGTGATACTAAAGAAG(+ACTCAC)GATCCACCCAGTGAGGGCGGGGTCTCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605000847_6bo7 CTATACCTAGACCACAGTTTGATCTGTAGCTG(+ACTCAC)GTACCTCATCAGAGCATAGCTCCGCCATCCC NGG 10 8 17 8 24 0 100 5 10
    240605000846_wpho CAGCAGGGATGGCGGAGCTATGCTCTGATGAG(+ACTCAC)GTACCAGCTACAGATCAAACTGTGGTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240605000845_rg1p GACCACCCCAGGTCTTGTGTGTTTTCTATAAG(+ACTCAC)GTACCAGAATAAATGGGAAGTTGATTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605000843_wemn ATTTGTTTTAAATCAACTTCCCATTTATTCTG(+ACTCAC)GTACCTTATAGAAAACACACAAGACCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605000842_h68n AGAAAGCGTGCGCTATTCTGACTAATGCCTTG(+ACTCAC)GATCCCTCATCCTACCCATGTTTACTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605000840_8y2k CAAATACAGAAGTAAACATGGGTAGGATGAGG(+ACTCAC)GATCCAAGGCATTAGTCAGAATAGCGCACGC NGG 10 8 17 8 24 0 100 5 10
    240605000838_q811 GAAAGAATTGGCATGTTTTTTAATTTGCAGGG(+ACTCAC)GATCCTTGTGAGCATCTAGATAGTCCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605000837_6is1 AAGAGAGAAGGGACTATCTAGATGCTCACAAG(+ACTCAC)GATCCCCTGCAAATTAAAAAACATGCCAATT NGG 10 8 17 8 24 0 100 5 10
    240605000835_ejnw CCAGGGCTTTAGGATTGCTGAGTGCTCCGGAG(+ACTCAC)GATCCTCACATACCCACTGTTACTGTTCACT NGG 10 8 17 8 24 0 100 5 10