Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000746_ksri AAATACAGTGTACTTGAAATATAAAAACAATG(+ACTCAC)GATCCCCAGCTAGAGAATACAGTTACAAATA NGG 10 8 17 8 24 0 100 5 10
    240605000745_jxpx GCTCTCCATCACTGCTCATCACAGAAAGGAAG(+ACTCAC)GTACCTGGCCTGAGGTCAGGGCCATCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605000743_3bmo CAGACAGAGGGATGGCCCTGACCTCAGGCCAG(+ACTCAC)GTACCTTCCTTTCTGTGATGAGCAGTGATGG NGG 10 8 17 8 24 0 100 5 10
    240605000741_ajkv ATTGGAAACCAACTGTAACTCCAGTTCCACAG(+ACTCAC)GATCCGATGCCCTCCTCTGGCCTCTGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605000739_vfvq CTGGTGCTTGCAGAGGCCAGAGGAGGGCATCG(+ACTCAC)GATCCTGTGGAACTGGAGTTACAGTTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605000738_ouoe ATTGGAAACCAACTGTAACTCCAGTTCCACAG(+ACTCAC)GATCCGATGCCCTCCTCTGGCCTCTGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605000736_wrzl CTGGTGCTTGCAGAGGCCAGAGGAGGGCATCG(+ACTCAC)GATCCTGTGGAACTGGAGTTACAGTTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605000735_vd5d CCGACTTGCCAGCAAGAACGACGCTGCAACAG(+ACTCAC)GATCCTTCCGCACACATTTACTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605000734_pnz7 AGTTAAGCTCTCCCAGTAAATGTGTGCGGAAG(+ACTCAC)GATCCTGTTGCAGCGTCGTTCTTGCTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605000732_r8nv CTAACTCCGCTGTGATCCAGAATGGGCAACTG(+ACTCAC)GTACCGCTCAGTAGTACATAAACCCCTTCAA NGG 10 8 17 8 24 0 100 5 10