Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000731_vr1h GTTTGTTGAAGGGGTTTATGTACTACTGAGCG(+ACTCAC)GTACCAGTTGCCCATTCTGGATCACAGCGGA NGG 10 8 17 8 24 0 100 5 10
    240605000730_0zwt GAGGAGACAGAAAAGGCAGTGGTGTCCACTAG(+ACTCAC)GATCCTCTGTCCACCACTTGAGATAAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605000728_64ig ACACCGTACCTTATCTCAAGTGGTGGACAGAG(+ACTCAC)GATCCTAGTGGACACCACTGCCTTTTCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605000727_cm23 CATTTGCAGAGTGGACCCAGCAGTGCCCACAG(+ACTCAC)GATCCTTATTCACGTATCTAAGTCTGATATT NGG 10 8 17 8 24 0 100 5 10
    240605000726_5abj CATAGAATATCAGACTTAGATACGTGAATAAG(+ACTCAC)GATCCTGTGGGCACTGCTGGGTCCACTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605000723_7cut AGTAGGACCATCCAGAAACTTCTTCACCCAGG(+ACTCAC)GATCCATCCCATAGTCAGCCACCAAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240605000721_pvor AGTGTCTGCATTTGGTGGCTGACTATGGGATG(+ACTCAC)GATCCCTGGGTGAAGAAGTTTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605000720_eipl CAGTGAAGTTCAGTCAAGTCAAAGGTCAATTG(+ACTCAC)GATCCCAATGTGGCTGCCTGAAAGGTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605000718_6wec TGTGGTAACAACCTTTCAGGCAGCCACATTGG(+ACTCAC)GATCCAATTGACCTTTGACTTGACTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240605000717_pzcc TATCTGTTTAGTCAAAACTCTGAGTGTGCTTG(+ACTCAC)GATCCCTCTGTTTGATCTGTCAAACTCTTCC NGG 10 8 17 8 24 0 100 5 10