Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000632_f3as ATATAATGTTTAGGTTCTAAGGAAGAATTGTG(+ACTCAC)GATCCCTAACAAACATCTTAGGAACATCTTT NGG 10 8 17 8 24 0 100 5 10
    240605000631_fe3f CAAATAAATTCTTAAACTTACCCACCAGTCTG(+ACTCAC)GATCCTTGGTAGTCATTGCACATTACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605000629_p3gm TTTGCGTGCTGTAATGTGCAATGACTACCAAG(+ACTCAC)GATCCAGACTGGTGGGTAAGTTTAAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240605000627_a7zq CCTTAATTTAAACATAGATACATGTCAGCCAG(+ACTCAC)GTACCATCCATGATGCAGGTGTTGATAATTA NGG 10 8 17 8 24 0 100 5 10
    240605000626_wlp9 TAAGTTAATTATCAACACCTGCATCATGGATG(+ACTCAC)GTACCTGGCTGACATGTATCTATGTTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605000624_p0dv CATGTTAATGTGGGTCTGCATCTCACCTGCTG(+ACTCAC)GATCCTGGGGTCCTGCACCTCACCTCTACAG NGG 10 8 17 8 24 0 100 5 10
    240605000621_0g46 GTGAACTGTAGAGGTGAGGTGCAGGACCCCAG(+ACTCAC)GATCCAGCAGGTGAGATGCAGACCCACATTA NGG 10 8 17 8 24 0 100 5 10
    240605000620_c4bw AGTTTAGTCACAGCGTAGGGGTGCTTCTGTGG(+ACTCAC)GTACCCAGCCTCAGCTGTGATCCTAAGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605000618_n3cw CACCCTACTCTTAGGATCACAGCTGAGGCTGG(+ACTCAC)GTACCCACAGAAGCACCCCTACGCTGTGACT NGG 10 8 17 8 24 0 100 5 10
    240605000616_e2fw AGCCAGCATGAATTCGTGTCCAGTTTTCCTGG(+ACTCAC)GATCCAAATTCTCAAGGTTATTTCTGAGTTG NGG 10 8 17 8 24 0 100 5 10