Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000614_xc4q AAGAACAACTCAGAAATAACCTTGAGAATTTG(+ACTCAC)GATCCCAGGAAAACTGGACACGAATTCATGC NGG 10 8 17 8 24 0 100 5 10
    240605000613_e34j ATGAGCTGAGAGGCCCTGCAGGGCCCTCCATG(+ACTCAC)GATCCATGCAGTTGGAGATGCTAATGCCTAT NGG 10 8 17 8 24 0 100 5 10
    240605000611_lcu2 TCCTGATAGGCATTAGCATCTCCAACTGCATG(+ACTCAC)GATCCATGGAGGGCCCTGCAGGGCCTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605000610_mvek ACTAAGACACTTTCCCTCAGCGTGTGACTCAG(+ACTCAC)GATCCTCTGTGTTTGAATTTCACATGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605000609_hqf9 ACCCTTCTCCCATGTGAAATTCAAACACAGAG(+ACTCAC)GATCCTGAGTCACACGCTGAGGGAAAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605000606_fxo8 CATACCTGAAATGTAAGTGAAGTCAGCCCATG(+ACTCAC)GATCCCAGAGCAGCCTCACTGTGCATGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605000605_z49w AGATCCCACCATGCACAGTGAGGCTGCTCTGG(+ACTCAC)GATCCATGGGCTGACTTCACTTACATTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605000603_8uuz ACATGAGCTCACCAGGAGCTGCTGTTTTCTGG(+ACTCAC)GTACCTTCTGTAGAGAACTTACCCATCACAT NGG 10 8 17 8 24 0 100 5 10
    240605000602_y3eu CACCCATGTGATGGGTAAGTTCTCTACAGAAG(+ACTCAC)GTACCCAGAAAACAGCAGCTCCTGGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605000601_y9jj CCTCTAAAGGAGGATTAAGAAGGTAAGAGTTG(+ACTCAC)GTACCTAACCTAGATATGGAAGACTTAGTCA NGG 10 8 17 8 24 0 100 5 10