Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000648_9nsi TCCAAGTGGTAGTGGCAACCACACCGAGAGAG(+ACTCAC)GATCCAGAAGGAGACACACATAAAACAGGTA NGG 10 8 17 8 24 0 100 5 10
    240605000646_ac2s TTATATATTGTCCATTAAACCCATGTTCCATG(+ACTCAC)GTACCTCACTGGAAGAATTGGGTCCTTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605000645_pq7r CCTTTAAGAAAGGACCCAATTCTTCCAGTGAG(+ACTCAC)GTACCATGGAACATGGGTTTAATGGACAATA NGG 10 8 17 8 24 0 100 5 10
    240605000644_ht9d AGAGCACACACACACAGCTCGTGTGCGTGCGG(+ACTCAC)GATCCTCTTCTACTCTCATCATCACATACTG NGG 10 8 17 8 24 0 100 5 10
    240605000642_9he9 GAACACAGTATGTGATGATGAGAGTAGAAGAG(+ACTCAC)GATCCCGCACGCACACGAGCTGTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605000640_ntyl GAGGACTTCCAGGAAGCCATATGGCTGCCCTG(+ACTCAC)GTACCAAGGAGTTGAGTTTACCATGAATACC NGG 10 8 17 8 24 0 100 5 10
    240605000638_lyqd GCTGCGGTATTCATGGTAAACTCAACTCCTTG(+ACTCAC)GTACCAGGGCAGCCATATGGCTTCCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605000636_qarx ACACAATCAAGATTGGTCACTGTACAACAAAG(+ACTCAC)GTACCTTTATGTGGCAGGGGCTAGGGACTGA NGG 10 8 17 8 24 0 100 5 10
    240605000635_8hyb TGGGTTCAGTCCCTAGCCCCTGCCACATAAAG(+ACTCAC)GTACCTTTGTTGTACAGTGACCAATCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605000633_3m1k TCCTAAAAGATGTTCCTAAGATGTTTGTTAGG(+ACTCAC)GATCCACAATTCTTCCTTAGAACCTAAACAT NGG 10 8 17 8 24 0 100 5 10