Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000542_2x0l ACAACCAGGCCCTAGGCCCCACTCTGAATGGG(+ACTCAC)GATCCTCCAAACTCCTCCAGCCCATCATATA NGG 10 8 17 8 24 0 100 5 10
    240605000540_h3ay TAGTAATAGAACAGTGATTAAGACAAAAGTTG(+ACTCAC)GTACCAAGGAGCAAAGTATTCTATGAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605000538_qs8i GTTAGGCTTTTCATAGAATACTTTGCTCCTTG(+ACTCAC)GTACCAACTTTTGTCTTAATCACTGTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605000537_uma2 ATGGATTTCTTACTCCTCTTCTCCATGGCTGG(+ACTCAC)GATCCTAGCTTCAGTCCTCATATCTGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605000535_4ul0 GGTGAGTAAACAGATATGAGGACTGAAGCTAG(+ACTCAC)GATCCCAGCCATGGAGAAGAGGAGTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605000534_cd1i TGCACACATGACATCTATGTAGCATATGCTAG(+ACTCAC)GATCCAGACGGACCTTAGAGTCTAAATCACC NGG 10 8 17 8 24 0 100 5 10
    240605000533_kku8 AGCATGGTGATTTAGACTCTAAGGTCCGTCTG(+ACTCAC)GATCCTAGCATATGCTACATAGATGTCATGT NGG 10 8 17 8 24 0 100 5 10
    240605000532_2jsr AGCACTTCACCTGCACGAAGGGATGAGACTGG(+ACTCAC)GATCCCTGAGTAAACTTCTTTAATAGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605000530_dwj7 ATAGCACACACTATTAAAGAAGTTTACTCAGG(+ACTCAC)GATCCCAGTCTCATCCCTTCGTGCAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605000529_83yn CTGGTTCCCATGGAAGCCAGAAGAGGGTGCTG(+ACTCAC)GATCCCTTGGAACTTGAGTTACAGACAGCTG NGG 10 8 17 8 24 0 100 5 10